Visualization of spatiotemporal distribution of ion concentration around spheroids by electronic impedance tomography method

Abstract

As a method for identifying cell types, a comb-shaped impedance sensor was used to quantify the ion flux via the ion channel by changing the ion concentration around the cell over time. However, in cancerous cells and asymmetric neurons, the ion concentration is spatially distributed, so it is necessary to capture it as a two-dimensional density image. In order to visualize the spatiotemporal distribution of ion concentration around cell, electrical impedance tomography (EIT) is carried out using a newly developed tiny multiple-electrode sensor. The developed tiny multipleelectrode sensor has 8 electrodes with a length of 0.40 mm and a width of 0.15 mm and 1.0 mm in sensing diameter. In experiment, ion concentration around cell spheroid which is constructed using WT type of MRC-5 cells is visualized by EIT on the tiny multiple-electrode sensor. As a result, it was confirmed that spatiotemporal distribution of ion concentration around the cell spheroid increased with the passage of time, and it is successfully visualized by EIT with the tiny multiple-electrode sensor.