超音波霧化を利用した細胞培養法によるマウス筋芽細胞の増殖

抄録

Cell culture method using ultrasonic atomization of culture medium is a recently proposed method under atmospheric pressure. In contrast to conventional method, this method can prevent cell desiccation, but the appropriate culture conditions are unknown. In order to improve this method, an environment for the proliferation of mouse myoblast cells was established using atomization culture. Initially, a cell culture device using piezoelectric element was fabricated, and experiments were conducted to evaluate the resonant frequency and input voltage. Based on the results of the experiments, the input frequency and input voltage were determined to be 2.04 MHz and 211.9 V, respectively. Subsequently, culture experiments were conducted under two conditions with burst ratios of 0.02 and 0.04. As a result of the experiments, it was confirmed that the cell area increased with burst ratio of 0.02 but decreased with burst ratio of 0.04. Furthermore, the surface of the culture base was divided into 10 areas, and the cell area in each area was compared, and it was confirmed that the area was generally uniform.