Cytokinesis requires substrate in adhered cells. However, it is obscure whether cells require external forces under the cytokinesis. In this study, external forces applied on cells during cytokinesis were attempted to measure with traction force microscopy. It was investigated whether single MDCK cells divide on polyacrylamide gels and whether FBS, which is required for stimulating cellular division, and phenol red, which is required for indicating pH, crosstalk with GFP-tubulin and with fluorescence beads.