抄録
In this study, in order to measure the softness of cultured tissue, ball indentation tests were performed. As first step, the principle of ball indentation test was applied to the thin silicone rubber sheet (t=0.1mm), which achieved results confirm the validation of the measurement result. Next step, Young's modulus of monolayer cultured nerve cell (PCI2) was measured, and the validation of the measured results was confirmed by comparing with previous studies. The Young's modulus of the cell body is 36.5kPa, and distal, middle and proximal of neuritis are 50.2kPa, 21.8kPa and 51.5kPa, respectively. In addition, Young's modulus of three-dimensional gel-culture system consisting of primary human gingival fibroblasts (GF) and gingival epithelial (GE) cells in collagen gels were measured. The Young's modulus of whole gel and gingival epithelial cells are 3.46kPa and 81.9kPa, respectively. The results were shown the possibility of measuring the Young's modulus of each layer of cultured tissue in the case of laminate structure.
