Abstract
Motor proteins, dynein and kinesin, are expected to be used as a nano actuator in vitro. To reconstruct the motor protein system based on gliding assay, one of challenging issues is to control the directionality of gliding microtubules. There have been several studies reported about the control using microfabricated structures. However, a microtubule was driven by only kinesin in reported systems, so that the microtubule moves unidirectionally with its minus-end as the leading head. Therefore, a molecular system, in which microtubules glide bidirectionally using multiple motors, is demanded for expanding the usability of motor proteins for engineering purposes. In this paper, we selectively modified the top glass surface of a flow cell with dynein, and the bottom with kinesin, and tried to select a surface which a microtubule attach to by electrophoresis. We evaluated the bidirectional movement of microtubules and control of direction by observing the leading.
