抄録
We have developed a micro-device that enables to measure the neural activity of cultured cells evoked by photo stimuli at a single-cell level spatial resolution. The device is designed as an in-vitro model for a novel implantable device that is used in our future research to clarify the detailed mechanism of neural diseases such as anxiety disorders and Parkinson disease based on optogenetics. The device is fabricated by patterning microstructures of a pinhole array and a microelectrode array on a planer glass substrate. The single-cell level photo stimulation is achieved by laser light transmitted through pinholes that are 5 μm in their diameters. In order to evaluate the applicability of the pinholes, we investigated the spot size and strength of laser light through the pinholes.
