Abstract
The Plasmodium of Physarum polycephalum is a unicellular and multinuclear giant amoeba. The cell body of the Plasmodium roughly consists of two parts, namely, sheet-like locomotive front and tubular rear part. The tubular structure of the Plasmodium is a kind of transportation network, and some studies have already been done on the structure and function of the plasmodial network. However, there are some difficulties in observing the minute structure of the plasmodial network. We thus developed a new method for the observation of the plasmodium that is a macroscopic implementation of dark-field microscopy.
