Abstract
Lignocellulosic biomass is expected to be utilized as one of the renewable energy. Lignocellulosic biomass can produce bioethanol from a series of processes; pretreatment, enzymatic hydrolysis and fermentation. However, fermentation inhibitors are generated during the process of pretreatment. Fermentation inhibitors which include Furfural, 5-Hydroxymethylfurfiiral (5-HMF), Acetic acid, Formic acid decrease the rate of growth of yeast. In this study, we analyzed the effect of these inhibitors on yeast growth. Moreover, in research on ethanol production from lignocellulosic biomass, the enzymatic Simultaneous Saccharificiaton and Fermentation (SSF) is desirable method in practical application. However, the optimum temperature of enzymatic saccharification is different from that of yeast fermentation. Therefore, we tried to analyze the effect of temperature on yeast growth. As a result, the growth rate at 40 ℃ and 45 ℃ was lower than that of the other temp. We also analyzed the effect of contact area of the air. We changed the size of flasks from 100 to 500 mL and measured of growth rate of yeast. The surface area is no effect on growth rate of yeast.
