Abstract
Hyperthermia and diathermy in the medical application of biothermal engineering expose cells and tissues to high-temperature stress, and damage and destroy them. Response of the cells to high-temperature stress is interesting not only in the medical application, but also thermal-biologically. This study investigated the behavior of cells in response to high-temperature stress to analyze influence of the temperature on the behavior as compared with common incubation-temperature of 37℃. Morphological change and mobility of cells were investigated by time-series three-dimensional imaging using a digital holographic microscope, which gave the thickness distribution of cells based on the information of phase difference in holography. Volume, projected area, average and maximum of thickness and position for every cell were in time-series quantified and analyzed. Therefore, the characteristic changes in cell-morphology including deformation of cells, formation of blebs from the cells and collapse of cell structure with the cell-volume decreasing, and the moving velocity of cells were revealed.
