Abstract
A method to enhancing supercooling is one of most promising techniques for cryopreservation. The object of this paper is screening of plant-derived compounds having supercooling-facilitating (anti-ice nucleation) activity. In this study, ice nucleation catalyzed by ice-nucleation bacteria (Xanthomonas campetris) was tested for six kinds of plant-derived compounds by using micro-droplet freezing assay. Firstly, the supercooling-facilitating activity was depicted for oxyresvertrol and taxifolin. Secondly, the activity of compounds does not depend on the number of hydroxyl site. It was found the activity increased with decreasing the molecular weight of compound. Thirdly, one-dimensional growth experiments were carried out to observe the pattern formation of ice/solution interface growing from taxifolin solution. The column structure of primary arm was observed in the leading front. The results suggests taxifolin prevent the specific surface of ice crystal.
