In this paper, an on-orbit calibration method for vibrating structure MEMS-gyroscopes for micro-satellites is proposed. The purpose is high-accuracy attitude determination with low power consumption sensors under tight restriction of electrical power. This research is based on a micro-satellite " TSUBAME " , which is being developed at Matunagalab, Titech/ISAS. If MEMS-gyroscopes are calibrated by fiber optic gyroscopes once, then the subsequent attitude of the satellite is determined accurately with low power consumption sensors during the normal operation. The effectiveness of this method is confirmed by the numerical simulation.