Ignition behavior of dual fuel HCCI combustion is affected by low temperature oxidation reaction. In this study, the behavior of low temperature oxidation reaction when iso-octane is mixed with n-heptane was investigated by engine experiments and chemical kinetic analysis. The engine experiments show that iso-octane apparently has little influence on heat release in low temperature reaction. From chemical analysis, this is because iso-octane reacts with OH radicals to suppress low temperature reaction of n-heptane while the low temperature reaction of iso-octane itself is activated to cover the loss of heat release in n-heptane reaction.