Abstract
Protein kinase C α (PKCα) in endothelial cell is activated by Ca^<2+> and diacylglycerol (DAG). Recently, our experimental study showed that activated PKCα was translocated to membrane site by various stimulations. In this study, to understand this mechanism, we developed a computational three-dimensional (3D) model of PKCα translocation based on our experimental data. In expreiments, we found a time lag between Ca^<2+> diffusion and PKCα translocation. We used RICS System (RIKEN) which can caliculate the biochemical reactions and molecules movement. We reconstacted 3D cell model from confocal microscope image data and built a biochemical mode with Ca^<2+>, PKCα, and DAG. We simulated this PKCα translocation with the RICS system, so that we will study various drugs stimulations.
