J0270203 Construction of Micro-environment For Function Analysis of Stem Cells : Efficient Manipulation of Single Cells by Combination of Microprobe Array and Microwell Array

Abstract

Cell manipulation system is an essential tool for reconstruction of cellular microenvironment that leads to high-throughput function analysis. In this paper, we developed a cell manipulator array integrating a cell trapping function on tips, which is a major part of the cell manipulation system. To transport cells electrically, an electrokinetic pump was fabricated and bonded to a PDMS sheet. This pump generated suction and ejection flows through a punched hole. The flow was characterized by measuring trajectory of fluorescent particles. We fabricated electrode pairs for dielectrophoretic cell manipulation. Cells were trapped in between the electrode pairs. We characterized viability of the trapped cells and studied the effect of AC voltages on cells.