抄録
The time-controlled atmospheric pressure plasma flow is generated and irradiated to the living-cell suspended solution for clarifying the transfection mechanism toward developing highly-efficient and minimally-invasive gene transfection system. It is observed that the transfection efficiency is significantly increased by the short-time (< 4 sec) and short-distance (< 40 mm) plasma irradiation, and the high transfection efficiency of 53% is realized together with the high cell viability (> 90%). This result indicates that the physical stimulation such as the electric field caused by the charged particles arriving at the surface of the cell membrane, and chemical stimulation associated with plasma-activated products in solution act synergistically to enhance the cell-membrane transport with low-damage.
