抄録
We propose a new measurement method for simultaneously measuring thickness and sound velocity for soft materials such as a conductive polymer film and stacked induced pluripotent stem cells (iPS cells). Using the 75 MHz underwater-type ultrasound transducer, simultaneous thickness and sound velocity measurement has been carried out by combining the acoustic resonant spectroscopy and pulse-echo measurement. The advantage of this method is that the thickness can be derived without using the known value of sound velocity. The thickness of the conductive polymer film and the stacked iPS cells on metal substrate are measured at 10.4 μm and 16.0 μm, respectively. The thickness of conductive polymer and the staked iPS cells agrees well with the reference measured by the confocal laser microscope. T These results indicate that the developed measurement method can be one of the potential candidates for the real-time thickness measurement for the soft materials. For future work, the thickness of living iPS cells should be measured in real time. Using this method, we would like to figure out the relationship between the stacked state and the differentiation of iPS cells.
