細胞損傷に及ぼす静水圧と温度の影響

抄録

Preservation method is studied to improve survival rate after preservation. In cryopreservation, cells are damaged by the growth of ice crystals. Therefore, using the phenomenon that freezing point of water becomes lower under high hydrostatic pressure, we investigated the method to preserve cells at subzero temperature by using hydrostatic pressure. As the experimental target, we chose eggs of medaka because it is easy to get and observe them. We observed the behavior of them under various temperature and pressure, and checked whether to hatch after experiment. As the result, we found that the path of pressuring and cooling had an effect to hatching rate. The hatching rate when pressuring after cooling is higher than pressuring after cooling. And longer the time of pressuring became, lower the hatching rate was. The lower pressuring rate improved the hatching rate. Utilizing hydrostatic pressure for preservation requires more long time experiments.