共培養のための多段モールド製法による多層ゲルチューブの構築

抄録

This paper describes a fabrication method of a multi-layer heterogeneous polymer gel tube device. The device can be easily fabricated by the repetition of a simple molding process, and the device works as an in vitro 3D engineered scaffold by encapsulating multiple types of cells during fabrication process. Thicknesses and the numbers of the layers can be flexibly designed by changing the mold size, and the perfusion culture can be easily performed by just connecting external pump system to silicone tubes of the device. We fabricated several different size and material types of devices, and succeeded in controlling coefficients of variation of the size to be lower than 5%. We succeeded in cultivating several types of cells in and on the collagen tubes. We believe that this method could help the easy reproduction of various complicated in vivo tissues, and contribute to the development of regenerative medicine and pharmacokinetic testing.