細胞動態計測に向けたグルコース脱水素酵素型マイクロセンサの開発

抄録

In medical and drug discovery studies, it is necessary to investigate efficacy of drug candidates under development. Conventionally, cell-based assays using human cells are widely carried out as an alternative to animal testing for this purpose in vitro. However, it is difficult to evaluate the effectiveness over time of drugs using conventional methods which are not capable of measuring cell kinetics. Currently, microfluidic devices integrated with a glucose sensor have been proposed for measurement of cell kinetics in the research field of μTAS (Micro Total Analysis Systems). Because cells consume glucose as an energy source, glucose consumption is an important indicator related to their activities. We aimed to develop a microfluidic device integrated with a glucose sensor which is based on glucose dehydrogenase (GDH) for online measurement of cell activities. In this study, we evaluated function of a GDH-based glucose sensor that we developed by comparing with a glucose oxidase (GOD)-based glucose sensor. Then, the sensor was integrated into a microfluidic device to investigate functions on flow rate dependency and glucose concentration. The results showed that glucose concentration can be measured with high-resolution and high sensitivity from current density obtained by the sensor, suggesting that it is possible to measure cell kinetics on-line on chips.