術中迅速免疫組織染色を実現させる電界撹拌技術の開発

抄録

We aim to develop innovative stirring technology (electric field mixing (EFM)) applied abrasive control technique with AC electric field. This technique allows for stirring without contact by applying AC electric field to droplets. This technique was applied to intraoperative immunohistochemical staining. Under EFM showed antigen-antibody reaction time in immunohistochemical staining can be shortened from 90 minutes of conventional method to 10 minutes. In this study, EFM examined the mechanism which makes antigen-antibody reaction of an immunohistochemical staining quicken. As a result, we obtained that it has the mechanism which a reaction time of an immunohistochemical staining is shorten because antigens and antibodies improve to increase the frequencies of contact, and further antibodies disperse and becomes easy to react to antigens under EFM. compared with conventional method.