Development of microfluidic device for hyphal elongation control and morphological observation of filamentous fungus Trichoderma reesei

Abstract

Monitoring of cultivation is important for efficient industrial enzyme production by filamentous fungi. The purpose of this study is to clarify the relationship between morphology of filamentous fungi and enzyme production, and to provide novel monitoring method for filamentous fungus cultivation. we developed a microfluidic device to observe the complex hyphal morphology of filamentous fungi in real time, and observe a hyphal growth from spores wonder enzyme producing condition.