Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : September 13, 2020 - September 16, 2020
We proposed a quadrupole capillary dielectrophoretic (DEP) device, which was a new DEP device enabling invasive and effective cell-separation without contacting the cell suspension to metal electrodes. The device consisted of four brass rods and a glass capillary. The brass rods were glued around the glass capillary to facilitate cell separation by generating a non-uniform AC electric field in the entire cross-section of the glass capillary. Prior to the experiment, we numerically simulated distributions of the electric potential, electric field, and the gradient of electric field square in the cross-section of the glass capillary. Predictions demonstrated that the maximum of the gradient of electric field square were appeared near the internal wall of the glass capillary, implying that p-DEP and n-DEP cells would be separated effectively due to the steep slope of the gradient of electric field square created in the radial direction. The effectiveness of the quadrupole capillary dielectrophoretic device was verified by conducting cell-separation experiments using live and dead human breast epithelial cancer cells (MDA-MB-231).
