微細加工基板による細胞核の局所的な圧縮はDNAの紫外線耐性を向上させる

抄録

Ultraviolet (UV) rays or radiation impose crucial damages to the intranuclear DNA, causing cell death and carcinogenesis. Therefore, researches focusing on the mechanism of DNA damage generation and repair is important. We previously found that the UV radiation-induced DNA damages were significantly suppressed in the nucleus compressed by using a microfabricated substrate (micropillar substrate) in which fine cylinders with a diameter and height of several μm are arranged. However, the detailed mechanism underlying the UV radiation resistance of DNA in the compressed nucleus has not been clarified yet. In this study, we investigated the detailed mechanism of the promotion of the UV radiation resistance of DNA caused by the nuclear deformation. The nucleus in the cells on the micropillar substrate manifested a significant resistance for UV radiation, especially on the micropillars with circular cross section. Detailed observation with confocal microscope revealed that intranuclear DNA concentration facilitated by the nuclear deformation with the circular micropillars and leads to improve the UV radiation resistance of DNA. Our finding may provide a new concept for improving the radiation resistance of cells.