肝スフェロイドを導入した多臓器生体模倣システムの機能評価

抄録

Recently, the usage of cell spheroids and organoids has been investigated to improve the biomimicry of microphysiological systems （MPSs）. However, MPSs with cell spheroids need to be formed using conventional culture vessels, and it is difficult to make spheroids uniformly and in large quantities. In this study, we developed a novel multi-organ MPS that enables cell spheroid formation and culture by placing honeycomb microwells （HMWs） at the bottom of the culture chamber. We conducted a functional comparison with HepG2 monolayer culture and spheroids using the MPS. The results showed that the spheroids made with HMW had improved function compared to the cells culture with monolayer.