Effect of competitive adsorption of serum proteins on microfluidic surfaces

Abstract

We developed a microfluidic device by stacking plastic films at intervals of 20 μm using a press process and utilizing the gaps between the films. In this study, we evaluated the interaction between the surface of the device and serum components in order to apply the device to dengue fever diagnosis by ELISA. In this study, we evaluated the interaction between the device surface and serum components for the application to dengue diagnosis by ELISA. Therefore, the antibodies were covalently coupled to the device surface by EDC/NHS coupling to prevent detachment. The surface-modified device succeeded in detecting serum in less than half the time of the conventional method.