顕微鏡下マイクロ引張試験機による単一細胞の機械的特性・及び接着力の同時評価

～微小管重合阻害した子宮頸癌由来 HeLa 細胞の力学的特性変化～

抄録

Mechanical properties of cancer cells are important for considering the behavior of cancer cells. However, few studies evaluate the mechanical properties and adhesion force of whole cells at the same moment. In this study, we evaluated stiffness and adhesion force of cancer cells (HeLa) by using an in-process micro tensile tester which we developed. To analyze the effect of the microtubule on the mechanical property and the adhesion force of cells, we carried out the tensile test to treated cells with colchicine which inhibits polymerization of the microtubule. As a result, the upper stiffness of treated cells (14.2 ± 7.1 nN/%strain) was larger than that of control cells (5.6 ± 3.5 nN/%strain). Additionally, the adhesion force of treated cells (472.7 ± 278.5 nN) was also larger than that of control cells (182.2 ± 94.0 nN).