フィルム積層型構造体のフィルム形状がウェル内流れとELISAに及ぼす影響

抄録

In response to the demand for rapid and sensitive testing to prevent the spread of infectious diseases, we have developed a bioanalytical device in which circular films are stacked at 20 μm intervals and rotated in a well. While higher sensitivity and speed were reported, the constant rotation of the circular film was reported to cause retention of molecules in the in-vessel flow. Therefore, it is necessary to design the shape of the film including the in-vessel flow as well as the formation of microfluidic channels by stacking the films. In this study, we proposed the use of notches in the disc-shaped film to promote circulation of the in-vessel flow, and evaluated the effect of the notches using finite element analysis. The results showed that the notches in the film promoted flow in the z-direction better than that in the circular film. The design focused on the flow by setting the size and number of notches as parameters.