Abstract
In recent years, it has become clear that exercise produces a variety of bioactive substances, called myokines, that help maintain healthy body functions. However, myokines are difficult to assess unambiguously because they are influenced by other hormones. Therefore, in this study, we established a simple in vitro experimental system for myokine research. In this experimental system, artificial skeletal muscle is cultured in different stretching patterns. By changing the structure of the skeletal muscle and the culture medium, we can study the effects of muscle movement patterns on myokine production. Our research group has succeeded in producing artificial skeletal muscle that simulates human muscle from mouse muscle cells. We have designed and developed a culture system to apply different stretch stimuli to the artificial skeletal muscle. Our goal is to quantitatively evaluate the relationship between the amount of myokine secretion and the muscle movement pattern by applying different stretching stimulus patterns to the artificial muscle and investigating the effects on the amount of myokine secretion.
