Preparation and mechanical characterisation of bone repair composites using calcium carbonate from waste oyster shell

Abstract

The possibility of using calcium carbonate, the main component of oyster shells, as a bone replacement material was investigated for the effective use of waste oyster shells from the Seto Inland Sea. The osteogenic differentiation of mouse preosteoblast cell line MC3T3-E1 in vitro study was confirmed. This study aimed to investigate the preparation of CaCO₃/gelatin scaffolds for in vivo study and mechanical characterization. We successfully prepared CaCO₃/gelatin scaffolds with appropriate stiffness for osteodifferentiation.