To clarify the relationship between the amount of sample acquired and the method of needle insertion in endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNA), we rapidly punctured a porcine liver with a needle and measured the mass of the sample acquired under -90 kPa negative pressure. The results showed that a puncture speed of 75 mm/s yielded larger amounts compared to 300 mm/s at a needle tip angle of 18°. Additionally, the amount acquired was greater with a needle tip angle of 90° compared to 18° at a puncture speed of 300 mm/s.
