This paper describes an experimental study on full-field stress separation from thermoelasticity and photoelasticity measurements and its application to estimation of stress intensity factor and the J-integral. Thermoelastic stress analysis (TSA) and photoelastic stress analysis (PSA) have been developed as full-field visualization methods of stress distribution. Only the sum of principal stresses can be measured by TSA, while only the difference of principal stresses can be measured by PSA. In this study, the hybrid stress separation measurement technique using both of these methods developed by the present authors was applied for determining distribution of all individual stress components in a center-cracked plate subjected to mechanical load. Stress intensity factor and the J-integral were calculated from the stress distribution. It was found that these fracture mechanics parameters can be evaluated with good accuracies by the present technique.