Abstract
In the present study, effects of iso-osmolar trehalose solutions on intact murine myeloma cells (sp2) during the freezing-thawing process are discussed. Intact sp2 cells suspended in iso-osmolar trehalose were installed in cryomicroscope and frozen at various cooling rates (-1℃/min, -90℃/min, -5℃/min) down to -80℃, followed by rapid thawing, 90℃/min. It was found that extracellular ice grains in trehalose solution did not mechanically suppress cells even at lowest cooling rate, -1℃/min. Further, only a seldom intracellular ice formation were observed in all cooling rate for iso-osmolar trehalose solution. However ice recrystalization was observed for all the conditions even at a fastest warming rate, 90℃/min.
