Abstract
Exposure to hypertonic solution during freezing is believed to be one of the major causes of the post-thaw cellular injury after freezing at low cooling rates. We have characterized the post-hypertonic survival at 23℃ as a function of hypertonic NaCl concentration, the time of exposure, and the rate of increase or decrease in NaCl concentration by using a newly developed perfusion technique. In the present report the same experiments are carried out at 0℃ and compared with the previous result at 23℃. The cell membrane permeability to water is also measured to explain the obtained result.
