The purpose of this study is to investigate the signal transduction process for the environment response. It has been shown by various researches that the supernatant as conditioned medium have various growth factor and effective substance for the cell adhesion and inducer for cell differentiations. In this study, we focused attention on the conditioned medium of low-temperature and high-pressure environment. The change in viability of cells under low-temperature and/or high-pressure condition derived supernatant was evaluated. The high-pressure condition was set by hydrostatic-pressure apparatus using controlled piston mechanism. The results suggested the presence of some environment-derived communicative matter, but not yet have proved.
