Abstract
A cell rotation system (CRS) has been developed to observe three dimensional (3D) morphology of cells precisely from their images obtained from various points of view. In this system, cell held with a micropipette under a microscope is rotated around the axis of the micropipette set perpendicular to the optical axis, while the pipette tip is kept in position based on the binarized image of the pipette tip. The displacement range of the pipette tip was narrow in the previous system. In this study, a lever mechanism was introduced to widen the displacement range. We also introduced a flexible cell access mechanism capable of holding an arbitrary cell on a slide glass. In addition, we have succeeded in the real-time pipette tip position control by using feedback control in combination with feedforward control.
