The stress relaxation test was performed for cultured rat aortic smooth muscle cells using a laboratory made micro tensile tester with feed-back control. Untreated cultured cells and cells treated with cytochalasin D to disrupt their actin filaments were stretched by 70-85%, and their length was kept constant to obtain stress relaxation curve. Viscoelastic analysis with a standard linear solid showed that viscoelastic parameters of the cells did not change in response to actin filament disruption. Fluctuation in tension was observed in the stress relaxation curve of the untreated cells. Such fluctuation disappeared in cells treated with cytochalasin D. The fluctuation in tension may be caused by dynamic change of intracellular actin filament organization.