Abstract
Micro-folding culture system is proposed where cells are cultivated on micropatterned substrates. The substrates have a number of pits with diameter of 100, 150 and 200μm, and has low cell-adhesiveness surface. Mesenchymal stem cells (MSCs) were seeded onto the patterned substrates at a density of 1.1×10^5 cells/cm^2. Aggregates of the MSCs were formed in the pits within 48 hours. Diameter of the aggregates and movement of aggregates had positive and negative correlation with diameter of pits respectively. There is a possibility that some function of aggregate can be controlled by the micro-folding culture.
