In developing artificial cartilage, made from poly(vinyl alcohol) hydrogel (PVA-H), it is important to develop a method for imparting cell attachment to certain areas of PVA-H surface because PVA-H itself has very poor biocompatibility. In this experiment, hydroxyapatite (HA) thin film 300nm thick was deposited by pulsed laser deposition technique on PVA-H. And the interaction between PVA-H and mouse fibroblast was examined by a cell culture method. The cell attachment on HA coated PVA-H (water content 33%) showed the maximum that was as high as commonly used tissue culture dish. Although higher water content (53%) PVA-H, this technique was effective in the improvement of cell attachment. Pulsed laser deposition is novel technique for quick and firm attachment between PVA-H and the underlying bone.
