Decellularization of biological tissue has been investigated using supercritical fluid extraction method. Carbon dioxide and fluoroform which have gentle critical conditions were applied as extraction media under various experimental conditions. Decellularization was evaluated by means of HE-stain and chemical quantitative analysis of DNA and phospholipids. Supercritical carbon dioxide that contained small amount of entrainer had a potential to extract phospholipids and nucleus without losing mechanical strength of tissue. In addition, decellularization with carbon dioxide could be attained within 30 minutes treatments. On the other hand, treatments with supercritical fluoroform alone caused slight hardening of tissue.
