Articular cartilage is charactericstic tissue which is mainly composed of avascular extracellular matrix. Solute transport in articular cartilage is to mainly by diffusion across the articular surface. We engineered regenerated cartilage by seeding bovine chondrocyte into agarose gel as scaffold. We evaluate diffusion coefficient by using fluorescent molecular with image process and to examine the effect of molecular weight and electric property of fluorescent labeled dextran on diffusion process in regenerated cartilage tissue.