Dermal collagen fiber plays an important role to determine morphological and functional property in the skin as a structural protein. An effective, optical probe method to investigate dermal collagen in vivo is a second-harmonic-generation (SHG) light induced by nonlinear optical property of collagen molecule. In this study, we demonstrated in vivo SHG imaging of dermal collagen fiber in human forearm skin using a mode-locked Cr: Forsterite laser with a center wavelength of 1250nm. The structural difference of dermal collagen fiber is observed along the depth direction. In vivo SHG imaging technique will be a powerful tool for the skin cosmetic and diagnostics.