3023 フェンス状ナノ構造配列を用いたDNA分離用マイクロ流路における分子の運動解析(J21 マイクロナノ理工学,J21 マイクロナノ理工学)

河合 雅弘; 多賀谷 洋一; 福澤 健二; 鈴木 健太; 馬場 雅和; 佐野 亨; 川浦 久雄; 張 賀東; 三矢 保永

doi:10.1299/jsmemecjo.2007.7.0_241

2007.7

Session ID : 3023

DOI https://doi.org/10.1299/jsmemecjo.2007.7.0_241

Conference information

3023 Analysis of Molecular Motion in Microchannel with Nanofence Matrix for DNA Separation

Masahiro Kawai, Yoichi Tagaya, Kenji Fukuzawa, Kenta Suzuki, Masakazu Baba, Tohru Sano, Hisao Kawaura, Hedong Zhang, Yasunaga Mitsuya

Author information

Keywords: DNA separation, Electrophoresis, Molecular trace, Micro total analysis systems, Nanostructured channel

CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract

We have analyzed motion of DNA molecules in the microchannel with nanofences in order to clarify the mechanism of size-based separation. The traces of DNA molecules were obtained from video images taken by a CCD camera with an image intensifier unit. We developed a program to trace DNA molecules in the microchannel during electrophoresis. It was found that smaller DNA molecules had longer detours around nanofences and the detour had nearly inverse relationship with applied voltage.

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