A new phase assigning algorithm for an automatic determination of the isochromatic parameter (relative fringe order) from a single color isochromatic image using fringe gradient and RGB wavelengths techniques is presented. This method uses the following three data: (1) the fractional phase retardation whose profile are of a saw-tooth type; (2) the points of reversal of increase or decrease in the fringe orders; and (3) the first unwrapped phase regions, which are used as seed points. The fractional phase retardation are obtained from a single color isochromatic image using fringe gradient technique. The points of reversal of increase or decrease in the fringe orders; and the first unwrapping phase regions are extracted from the isochromatic image using RGB wavelengths technique. To evaluate the effectiveness of the proposed method, a single color isochromatic image for a circular ring subjected to a concentrated load was used. Results showed that the proposed method yields the accurate relative fringe orders of isochromatics.