The influences of hydrogen on nano-indentation of IF-steel were investigated. The results obtained are as follows. (1) The "pop-in" was observed in the hydrogen uncharged specimen. (2) The "pop-in" was not observed in the hydrogen charged specimen. (3) The "pop-in" was observed in the specimen which heat treated at 200℃/1h. (4) The "pop-in" was observed in the specimen which charged with hydrogen after the heat treatment at 200℃/1h.