A tensile test up to 10% have been performed of an iron polycrystalline plates and strain distribution has been investigated using the digital image correlation analysis methods. Lattice rotation after the tensile test has been investigated by electron beam back-scattering pattern (EBSP) method. Strain distribution can be obtained with spatial resolution of as high as 10 μm and strain resolution of 1%. It has been found that one grain separates into several regions where different slip systems are activated. The subdivision of grains is found to occur by the lattice rotation around the <1,1,0>axis, which is brought about by the activation of different slip systems in adjacent regions separated by a (0,0,1) plane.