There are growing demands for determining the stress-strain relationship at a local area of materials since such knowledge on mechanical properties are indispensable for making a precise CAE analysis of MEMS as well as industrial materials systems. In this work, a load-controlled nanoindentation technique with a spherical tip has been applied to the determinations of stress-strain curves and the Yield stress for a low carbon steel surface. A multiple continuous indentation method is used for measuring load-penetration depth curves. It has been found that the estimated stress-strain curves and the Yield stress are almost agree with those measured by tensile tests when the influence of the residual stress of the specimen surface is taken into account.