Abstract
Stem cells play important role in tissue engineering. However, stem cells are very rare and difficult to identify. Therefore, an efficient and accurate cell separation method is needed. In this study, we developed a cell sorting system using a cone-plate rheometer for extraction of rare cells from cell mixture. The separation principle of this system is based on specific adhesion by the antigen-antibody interaction between cells and antibody-coated plate. We evaluated the performance of this cell sorting system using two different cells (HL-60, HUVEC) and antibodies (CD15, CD31).
