533 Construction of algorithm for motion analysis of kinesin-driven microtubules towards development of micro-transport devices
Details
Microtubules (MTs) gliding on the kinesin-coated surface in vitro have a good feasibility for development of micro-scale transport systems. The key requirements in the development of the system include an image analysis to detect and recognize gliding MTs. In this study, we present an algorithm for detecting the position of the leading tips of gliding MTs. The algorithm operates on binarized fluorescent images of MTs and extracts the tips of MTs with a kernel. For verification, the algorithm was applied to a sample segment, and the distance between extracted coordinates with the algorithm and given coordinates were referred to an error. As a result, the algorithm caused the error, in which the measurement length was shorter than the actual length by only a half of the sample width. By applying the algorithm to the gliding MTs, the tips were well extracted following the trajectories of gliding MTs. The present algorithm for the tip-tracking may be useful for constructing nano-scale transport systems.
