534 Influence of base length on template-directed circularization of ssDNA

Abstract

"Bottom up" is another possible approach to nanotechnology as well as the conventional "top down" approach. This approach requires special nano-structural elements with a size of nanometers and a property of self-assembly to an elaborate product. One strong candidate for the materials of such elements is single-stranded DNA (ssDNA), because this material possesses strict base-pair specificity and selectivity. In the course of fabrication of DNA nanostructures, circular single-stranded DNA (cssDNA) is often used as the intermediate form. The cssDNA is constructed by the simple Dolinnaya's method, which uses two of ssDNA, a body of the ring (Precursor) and a mediator that brings the DNA ends together (Template). Precursor was certainly circularized by Template and a ligating enzyme, and formed the monomeric cssDNA in a topoisomeric fashion, but multimeric cssDNA is also produced which was constructed from many precursors. The amount of monomeric cssDNA and multimeric cssDNA were altered by the length and sequence of the precursor and template.