J0401-4-4 Effect of Electric Field Stimulation on Chondrocyte Activity

Abstract

It is necessary for the regenerated cartilage to have same mechanical property as natural cartilage in a short period. The purpose of this study is to examine the effect of pulse wave electric field stimulation on activity of chondrocytes. Effect of pulse width, electric field value and frequency were evaluated to determine suitable condition for the stimulation. Isolated chondrocytes from porcine articular cartilage suspended with culture medium, and they were seeded in a custom made culture chamber that has parallel platinum electrodes. Chondrocytes were cultured for two days with electric field stimulation in an incubator. After the cultivation, amount of chondroitin sulfate was measured with DMMB method.